Designing PCR Primers on the Web

Designing PCR primers on the Web.

Designing PCR Primers Painlessly†

Amplicon: software for designing PCR primers on aligned DNA sequences

SUMMARY Amplicon is a program for designing PCR primers on aligned groups of DNA sequences. The most important application for Amplicon is the design of 'group-specific' PCR primer sets that amplify a DNA region from a given taxonomic group but do not amplify orthologous regions from other taxonomic groups. AVAILABILITY Amplicon is freely available as a script that will run on any platform wi...

PCR with arbitrary primers

PrecisePrimer: an easy-to-use web server for designing PCR primers for DNA library cloning and DNA shuffling

PrecisePrimer is a web-based primer design software made to assist experimentalists in any repetitive primer design task such as preparing, cloning and shuffling DNA libraries. Unlike other popular primer design tools, it is conceived to generate primer libraries with popular PCR polymerase buffers proposed as pre-set options. PrecisePrimer is also meant to design primers in batches, such as fo...